Primer3 Input -

SEQUENCE_ID=TP53_exon7_test SEQUENCE_TEMPLATE=GGACCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCTGTGACTTGCACGGTCAGTTGCCCTGAGGGGCTGGCTTCCATGAGACTTCAT PRIMER_TASK=pick_pcr_primers SEQUENCE_TARGET=120,1

SEQUENCE_INTERNAL_OLIGO_MIN_SIZE=18 SEQUENCE_INTERNAL_OLIGO_OPT_TM=55.0 Save this as my_primers.txt : primer3 input

Today, we are tearing down the primer3_core input file. Primer3 input is plain text. It uses a simple KEY=VALUE syntax. The engine reads these parameters, processes the sequence, and spits out the best primers. processes the sequence