Pgps3 Unique Restriction Sites Now

However, successful cloning depends entirely on one thing: knowing which restriction enzymes cut once —and only once—in your plasmid.

If you work with plant transformation, particularly in Arabidopsis thaliana or related species, you’ve likely encountered the PGPS3 vector. Derived from the pGreen series, PGPS3 is a compact, high-copy-number binary vector known for its small size (approximately 3.2 kb), making it ideal for golden gate cloning, site-directed mutagenesis, and efficient transformation into Agrobacterium tumefaciens . pgps3 unique restriction sites

| Restriction Enzyme | Position (approx.) | Recognition Sequence | Best Use Case | |-------------------|-------------------|----------------------|----------------| | | 1,234 | G^AATTC | Insert cloning (5' end) | | BamHI | 1,567 | G^GATCC | Insert cloning (3' end) | | HindIII | 2,345 | A^AGCTT | Backbone linearization | | XbaI | 789 | T^CTAGA | Subcloning from other vectors | | SacI | 2,890 | GAGCT^C | Terminator swaps | | PstI | 3,101 | CTGCA^G | Rare cutter for large inserts | | KpnI | 456 | GGTAC^C | Directional cloning with SacI | | NotI | 2,567 | GC^GGCCGC | For GC-rich inserts (rare cutter) | However, successful cloning depends entirely on one thing:

Just remember: no SalI, no EcoRV, and always verify your map. When in doubt, a quick virtual digest takes 30 seconds and saves three days of failed ligations. | Restriction Enzyme | Position (approx